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Detection of inactive precursors of β‐glucanases in Saccharomyces cerevisiae
Author(s) -
Hernandez Luis M.,
Olivero Isabel,
Larriba German
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)81005-9
Subject(s) - saccharomyces cerevisiae , chemistry , biochemistry , yeast , saccharomyces , microbiology and biotechnology , biology
Accumulation and secretion of β‐glucanases have been studied in vivo by using a thermosensitive secretory mutant of Saccharomyces cerevisiae blocked at the endoplasmic reticulum level (sec 18‐1). When incubated at the restrictive temperature no accumulation of active glucanases was observed. Following a shift to permissive conditions in the presence of cycloheximide a rise in the internal activity took place. The increase in total glucanase activity was partially due to the activation of an exo‐glucanase that hydrolyzes PNPG. It is concluded that glucanases are synthesized in inactive precursor forms and are converted to the active forms in their secretory pathway.