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Monoclonal antibodies for the detection and quantitation of the endogenous plant growth regulator, abscisic acid
Author(s) -
Mertens R.,
Deus-Neumann B.,
Weiler E.W.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80980-6
Subject(s) - abscisic acid , monoclonal antibody , radioimmunoassay , chemistry , endogeny , conjugate , regulator , plant growth , biochemistry , antibody , microbiology and biotechnology , biology , botany , gene , mathematical analysis , mathematics , immunology
Monoclonal antibodies (mAB) have been produced which recognize the physiologically active 2‐ cis ‐( S ‐form of the endogenous plant growth regulator, abscisic acid (ABA). Cross‐reaction with the ABA‐catabolites, phaseic and dihydrophaseic acid, is negligible, and ( R )‐ABA, 2‐ trans ‐ABA, the ABA‐conjugate, ABA‐β‐D‐glucopyranosyl ester, as well as the putative ABA precursor, xanthoxin, are totally unreactive. In addition to being very specific, the mAB exhibit high affinites for 2‐ cis ‐( S )‐ABA: the K values were 7.9 × 10 9 l/mol and 3.7 × 10 9 l/mol for antibodies from two different clones. By mAB‐radioimmunoassay (RIA), 4 pg 2‐ cis ‐( S )‐ABA (99.5% confidence level) can be detected. mAB‐RIA can be used to quantitate ABA directly in unprocessed plant extracts.