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Effect of pertussis toxin on hormonal responsiveness of rat hepatocytes
Author(s) -
Pushpendran Choliparambil K.,
Corvera Silvia,
Adolfo García-Sáinz J.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80966-1
Subject(s) - pertussis toxin , toxin , hormone , chemistry , endocrinology , medicine , biology , biochemistry , g protein , receptor
The ureogenic action of epinephrine in hepatocytes from normal adult rats is mediated through activation of α 1 ‐adrenoceptors. β‐Adrenoceptors in addition to α 1 ‐adrenoceptors, became involved in mediating this effect in cells from animals treated with pertussis toxin. The accumulation of cyclic‐AMP in response to epinephrine or isoproterenol was markedly increased in hepatocytes from pertussis‐treated rats as compared to that observed in control cells. The accumulation of cyclic‐AMP due to glucagon was also increased. It is suggested that pertussis toxin may release a constraint on adenylate cyclase activity by blocking the inhibitory coupling mechanism ( N i ) or some other entity involved in the regulation of the activity of this enzyme.