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Calcium uptake during the cell cycle of Saccharomyces cerevisiae
Author(s) -
Saavedra-Molina Alfredo,
Villalobos Rafael,
Borbolla Miguel
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80965-x
Subject(s) - saccharomyces cerevisiae , calcium , chemistry , biochemistry , cell cycle , microbiology and biotechnology , yeast , cell , biology , organic chemistry
Synchronous culture of the budding yeast Saccharomyces cerevisiae was obtained by sucrose density gradient selection with 90–100% of yeast synchronized by using the cells in the bottom. In these adult cells bud emergence is coincident with an increase in calcium uptake at 100 min of the culture, followed by a return to basal values which are maintained until the end of the first cell cycle of study. The phenothiazine derivatives, trifluoperazine and chlorpromazine inhibit bud emergence and trifluoperazine also increases calcium uptake.