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Metabolic activation of 1,2‐dibromoethane to a free radical intermediate by rat liver microsomes and isolated hepatocytes
Author(s) -
Tomasi A.,
Albano E.,
Dianzani M.U.,
Slater T.F.,
Vannini V.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80964-8
Subject(s) - microsome , chemistry , biochemistry , microsoma , enzyme
A one‐electron reductive metabolism of 1,2‐dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, under hypoxic conditions, it has been possible to trap a free radical intermediate and identify it by using 13 C‐DBE. Inhibition experiments have demonstrated that the site of activation is the microsomal drug metabolizing system.