Premium
Interaction of trifluoperazine with porcine calmodulin 19 F NMR and induced CD spectral studies
Author(s) -
Shimizu Toru,
Hatano Masahiro
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80962-4
Subject(s) - trifluoperazine , total factor productivity , chemistry , calmodulin , stereochemistry , analytical chemistry (journal) , calcium , chromatography , organic chemistry , productivity , economics , macroeconomics
We have used 19 F NMR and CD spectra to study interactions of TFP with CaM under various conditions where no aggregation of TFP occurs. It was found that TFP is bound to CaM in the absence of Ca 2+ with K a >4 × 10 4 M in terms of 19 F NMR of TFP and that KCl markedly influences the interaction of TFP with CaM. It was also found that TFP causes quite strong induced CD bands corresponding with the absorption bands of the aromatic ring of TFP when TFP is bound to Ca 2+ ‐CaM. The induction of the CD bands was time‐dependent and was composed of fast and slow phases.