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Fucosyl transferase activity and fucose incorporation in vivo as markers for subfractionating cucumber microsomes
Author(s) -
Sturm A.,
Kindl H.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80958-2
Subject(s) - golgi apparatus , fucose , vesicle , organelle , biochemistry , glycoprotein , chemistry , biology , microbiology and biotechnology , endoplasmic reticulum , membrane
Golgi apparatus only rarely seen in electromicroscopic examinations of post‐germinative storage tissues such as cucumber cotyledons could be detected by biochemical means. Enzyme activity transferring the L‐fucosyl moiety from GDP fucose to membrane‐glycoproteins was almost exclusively confined to golgi vesicles. This was demonstrated by comparing highly purified preparations of the ER, golgi and other organelles. The findings in vitro were supplemented by feeding experiments in vivo, establishing vesicles of golgi and plasma membrane as the by far dominating intracellular sites characterizable by fucose‐containing glycoproteins. Detailed analysis including the single peptides within the fractions revealed that the ER can be easily distinguished from golgi apparatus on the basis of glycoproteins.