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The effect of oligomycin on rat liver mitochondria respiring in state 4
Author(s) -
Masini Alberto,
Ceccarelli-Stanzani Daniela,
Muscatello Umberto
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80953-3
Subject(s) - oligomycin , respiration , mitochondrion , steady state (chemistry) , biochemistry , biophysics , titration , chemistry , biology , enzyme , anatomy , inorganic chemistry , atpase , organic chemistry
It has been found that oligomycin inhibits up to at least 50% state‐4 mitochondrial respiration. A time dependence of oligomycin inhibition has been shown. A titration curve for state‐4 respiration of sigmoidal profile has been presented. The possibility of misreading this oligomycin effect, so far never reported, has been excluded by evaluating the quality of mitochondrial preparations used in respect to their morphological, functional and electrochemical properties. The conclusion has therefore been put forward that the most part of respiration in steady‐state‐4 is driven by ATP synthesis.