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Sequence‐specific inhibition of RNA elongation by actinomycin D
Author(s) -
Aivasashvilli V.A.,
Beabealashvilli R.Sh.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80950-8
Subject(s) - elongation , nucleotide , rna , dna , sequence (biology) , nucleic acid sequence , dactinomycin , chemistry , microbiology and biotechnology , biology , biochemistry , gene , materials science , ultimate tensile strength , metallurgy
We have developed a method to localize specific sites where RNA elongation is arrested due to DNA‐bound ligands. The method was used to determine apparent binding sites for actinomycin D. We have found 14 strong RNA hindrance sites along nucleotide sequence of T7 and D111 T7 DNA of 380 nucleotides full length under low actinomycin D concentration conditions. Nucleotide sequence of all the sites is described by general formula XGCY where X ‡ G and Y ‡ C.