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Cellular RNA is not degraded in interferon‐treated HeLa cells after poliovirus infection
Author(s) -
Muñoz Alberto,
Harvey Robert,
Carrasco Luis
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80942-9
Subject(s) - hela , poliovirus , rna , interferon , microbiology and biotechnology , protein biosynthesis , nuclease , lysis , biology , ribosomal rna , reticulocyte , lymphoblast , virology , cytolysis , streptolysin , cell culture , chemistry , virus , biochemistry , cell , dna , cytotoxicity , in vitro , gene , bacterial protein , genetics
A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32 P‐labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus‐infected cells plus or minus interferon treatment, followed by translation in a reticulocyte lysate indicates that cellular mRNAs remain active. These results suggest that gross degradation of cellular RNA does not occur in interferon‐treated poliovirus‐infected HeLa cells and that a non‐specific nuclease induced by 2′–5′ A is not responsible for the inhibition of protein synthesis observed.