Premium
Isolation of deuterated histones from yeast grown on media dissolved in 2 H 2 O
Author(s) -
Hamana Koei,
Mita Kazuei,
Ichimura Sachiko,
Zama Mitsuo,
Kaji Keisuke,
Niimura Nobuo
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80928-4
Subject(s) - deuterium , yeast , chemistry , isolation (microbiology) , histone , radiochemistry , nuclear chemistry , biochemistry , biology , physics , microbiology and biotechnology , dna , nuclear physics
We have succeeded in growing Saccharomyces cerevisiae (baker's yeast) on media containing 2 H 2 O and isolating the core histones highly deuterated in the non‐exchangeable positions. The deuterated histones obtained here are of great value for their possible widespread use for structural studies of chromatin.