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Inhibition of Forskolin‐stimulated cardiac adenylate cyclase activity by short‐chain alcohols
Author(s) -
Robberecht Patrick,
Waelbroeck Magali,
Chatelain Pierre,
Jean-Claude Camus,
Jean Christophe
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80904-1
Subject(s) - forskolin , adenylate kinase , cyclase , stimulation , chemistry , medicine , ethanol , endocrinology , methanol , biochemistry , biology , enzyme , organic chemistry
The diterpene forskolin stimulated rat cardiac adenylate cyclase activity at least 20‐fold and potentiated the effect of NaF. The stimulatory effect of forskolin was reduced in the presence of Gpp(NH)p. Ethanol markedly reduced the stimulation of adenylate cyclase by forskolin while potentiating NaF and Gpp(NH)p stimulation. The inhibitory effect of ethanol on forskolin stimulation appeared to be of a mixed type with both a competitive and a non‐competitive component. Three other short‐chain linear alcohols (methanol, propanol, butanol) also inhibited forskolin‐stimulation, this effect being proportional to the number of carbon atoms.