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Characterization of sulphydryl groups of the mitochondrial phosphate translocator by a maleimide spin label
Author(s) -
Houstek J.,
Bertoli E.,
Stipani I.,
Pavelka S.,
Megli f.M.,
Palmieri F.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80900-4
Subject(s) - maleimide , spin label , chemistry , oxalate , mitochondrion , biophysics , ruthenium red , phosphate , biochemistry , membrane , stereochemistry , calcium , inorganic chemistry , biology , organic chemistry
A maleimide spin label strongly inhibits the phosphate/H + symporter of rat liver mitochondria. While inducing half‐maximal inhibition of transport, the spin label reacts preferentially with the SH groups of the carrier, which are at least of two types. One type of SH group is localized close to the surface of the membrane and its environment does not significantly influence the mobility of the probe. The second type of SH group is buried in the membrane, is not accessible to ascorbate or chromium oxalate and its environment greatly restricts the motion of the probe.