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The presence of LHRH‐like receptors in Dunning R3327H prostate tumors
Author(s) -
Hierowski Marion T.,
Perla Altamirano,
Redding Tommie W.,
Schally Andrew V.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80881-3
Subject(s) - dissociation constant , chemistry , receptor , antagonist , agonist , endocrinology , medicine , membrane , binding site , prostate , biology , biochemistry , cancer
Quantitative analyses of LH‐RH‐like membrane receptors were performed in five tumors from the transplantable Dunning R3372H rat prostatic adenocarcinoma. The binding of D‐Trp 6 ‐LH‐RH, an agonist of LH‐RH, was observed in all 5 tumors. The antagonist [Ac‐Dp‐Cl‐Phe 1,2 ,D‐Trp 3 ,D‐Lys 6 ,D‐Ala 10 ]‐LH‐RH was bound to 4 tumors. The apparent equilibrium dissociation constant ( K d ) for D‐Trp 6 ‐LH‐RH receptor was from 2.6–3.9 × 10 −10 M. The apparent equilibrium B max values (maximum number of binding sites) were from 17.2–86.0 fmol/mg membrane protein for D‐Trp 6 ‐LH‐RH receptor. The K d for the antagonist was from 2.4–2.7 × 10 −10 M and the B max values were from 35.5–66.0 fmol/mg membrane protein. Similar binding studies performed in 6 normal rat prostates showed no binding capacities.