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Cellular localization of the receptor‐dependent and receptor‐independent uptake of human LDL in the liver of normal and 17α‐ethinyl estradiol‐treated rats
Author(s) -
Harkes Leen,
Berkel Theo J.C.van
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80878-3
Subject(s) - endocrinology , receptor , medicine , ldl receptor , low density lipoprotein , in vivo , parenchyma , human liver , cell , lipoprotein , chemistry , biology , cholesterol , in vitro , biochemistry , botany , microbiology and biotechnology
The cellular localization in the liver of the receptor‐dependent and ‐independent uptake of human low density lipoprotein (LDL) in normal and 17α‐ethinyl estradiol‐treated rats was investigated by the simultaneous in vivo injection of human 131 I‐LDL and human reductive methylated 125 I‐LDL. The cells were subsequently isolated by a low temperature method. In untreated rats, after 30 min of in vivo circulation of human LDL, 57% of the receptor‐dependent liver‐association of human LDL occurs in non‐parenchmal cells and 43% in parenchymal cells. Estradiol treatment of rats for 3 days selectively increases the receptor‐dependent cell‐association of human LDL with hepatocytes (17‐fold), while the receptor‐dependent cell‐association with non‐parenchymal cells is not affected.