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Effect of elongation factor Tu on the conformation of phenylalanyl‐tRNA Phe
Author(s) -
Riehl N.,
Giegé R.,
Ebel J.P.,
Ehresmann B.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80871-0
Subject(s) - phosphodiester bond , transfer rna , ternary complex , chemistry , stereochemistry , nucleic acid , elongation , ef tu , conformational change , molecule , elongation factor , crystallography , biochemistry , rna , organic chemistry , enzyme , materials science , ultimate tensile strength , metallurgy , gene , ribosome
Structural features of the tRNA Phe molecule upon ternary complex formation with the bacterial elongation factor Tu were investigates. Phosphodiester bonds at positions 18 and 34 were found to be labilized in bound tRNA. Conversely, a higher stability of the phosphodiester links at positions 20, 21 and 36 was detected. Using ethylnitrosourea as a chemical probe a conformational change occurring at phosphate position 53 was observed in complexed tRNA. These results are interpreted by a structural rearrangement of the nucleic acid induced by complex formation.