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Part of the 23S RNA located in the 11S RNA fragment is a constituent of the ribosomal peptidyltransferase centre
Author(s) -
Barta Andrea,
Kuechler Ernst
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80844-8
Subject(s) - fragment (logic) , 23s ribosomal rna , rna , ribosomal rna , chemistry , ribosome , microbiology and biotechnology , stereochemistry , biochemistry , biology , computer science , gene , algorithm
Upon irradiation, 3‐[4‐benzoylphenyl]propionyl‐PhetRNA bound to the P‐site of poly(U)‐primed ribosomes is exclusively cross‐linked to 23S RNA. It is shown that the photoreaction only occurs with pyrimidine nucleotides. The site of the cross‐link is located within an 11S RNA fragment, which comprises the 1100 nucleotides at the 3′‐end of 23S RNA. The cross‐linked Phe tRNA derivative is still functionally active in peptide bond formation. The site labelled on the 11S fragment is therefore an integral part of the peptidyltransferase centre.