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Diazepam inhibits cell respiration and induces fragmentation of mitochondrial reticulum
Author(s) -
Vorobjev Ivan A.,
Zorov Dmitry B.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80842-4
Subject(s) - diazepam , mitochondrion , respiration , fragmentation (computing) , glutamate receptor , chemistry , cellular respiration , cell , microbiology and biotechnology , inhibitory postsynaptic potential , biochemistry , biology , pharmacology , endocrinology , anatomy , receptor , ecology
Diazepam (70–150 μg/ml) significantly inhibits oxygen consumption by pig kidney embryo cells and causes the cellular ATP level to fall. The maximum inhibitory effect develops after 1.5–2.5 h of diazepam treatment. In isolated mitochondria diazepam inhibits respiration in state 2 and 3 u with glutamate and in state 3 u with succinate. Ethylrhodamine staining and electron microscopic study reveal fragmentation of mitochondria in living cells.