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Sarcolemmal phospholamban is phosphorylated in isolated rat hearts perfused with isoprenaline
Author(s) -
Huggins John P.,
England Paul J.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80839-4
Subject(s) - phospholamban , isoprenaline , sarcolemma , phosphorylation , chemistry , endocrinology , medicine , calcium , inotrope , perfusion , contractility , biology , biochemistry , myocyte , stimulation
Phosphorylation of phospholamban in cardiac sarcolemma is implicated in the increased influx of Ca 2+ through the slow calcium channel induced by catecholamines. A method is described for the preparation of highly purified sarcolemmal vesicles from rat heart, and this has been used to examine the phosphorylation of phospholamban in 32 P i ‐perfused rat hearts. Phospholamban phosphorylation is increased 3‐fold after 30 s of perfusion with 0.1 μM isoprenaline. The time course of this increase precedes the inotropic response by 5–10 s.