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‘Cap 90’, a 90‐kDa Ca 2+ ‐dependent F‐actin‐capping protein from vertebrate brain
Author(s) -
Isenberg G.,
Ohnheiser R.,
Maruta H.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80824-2
Subject(s) - actin , protein filament , agarose , chemistry , affinity chromatography , cytoskeleton , microbiology and biotechnology , actin binding protein , biophysics , biochemistry , biology , actin cytoskeleton , enzyme , cell
A Ca 2+ ‐dependent actin filament‐capping protein of 90 kDa was purified from bovine brain using a new and rapid isolation procedure. This basically includes affinity purification on DNase‐I agarose. The protein caps the fast‐growing end of actin filaments but has no fragmenting or severing activity. Using Triton X‐100‐extracted cytoskeletons, capping and severing activities of actin‐binding proteins become clearly distinguishable from each other.