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Stimulation of de novo methylation following limited proteolysis of mouse ascites DNA methylase
Author(s) -
Adams R.L.P.,
Burdon R.H.,
McKin K.,
Rinaldi A.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80817-5
Subject(s) - proteolysis , methylation , enzyme , dna , dna methylation , stimulation , biochemistry , biology , microbiology and biotechnology , dna synthesis , methyltransferase , chemistry , ascites , gene , gene expression , endocrinology , medicine
The ability of mouse Krebs II ascites cell DNA methylase to add methyl groups to native, unmethylated DNA (de novo activity) is stimulated by limited proteolysis. The affinity of the enzyme for DNA is not altered by this treatment but the rate of reaction is increased so that 40% or more of methylatable sites are methylated within 4.5 h. The activation is associated with a decrease in size of the enzyme to 6.2 S.