Premium
Two site‐specific endonucleases Bin SI and Bin SII from Bifidobacterium infantis
Author(s) -
Khosaka Toshiyuki,
Kiwaki Mayumi,
Rak Bodo
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80812-6
Subject(s) - isoschizomer , restriction enzyme , bin , chemistry , dna , bifidobacterium , microbiology and biotechnology , biology , biochemistry , mathematics , lactobacillus , dna methylation , gene , gene expression , algorithm , fermentation
Two site‐specific endonucleases, Bin SI and Bin SII, were isolated from Bifidobacterium infantis S76e. Bin SI was found to be an isoschizomer of Eco RII, while Bin SII was shown to have the same sequence and cutting specificity as Bbe I, 5′‐GGCGCC‐3′. Both Bin SII‐ and Bbe I‐generated DNA fragments could be ligated with Hae II‐generated DNA fragments.