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Evidence for the presence of N 2 ‐(1,3‐dicarboxypropyl)‐L‐amino acids in crown‐gall tumors induced by Agrobacterium tumefaciens strains 181 and EU6
Author(s) -
Chang Chi-Cheng,
Chen Chong-Maw
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80802-3
Subject(s) - agrobacterium tumefaciens , asparagine , amino acid , glutamine , gall , strain (injury) , stereochemistry , chemistry , biochemistry , agrobacterium , biology , botany , gene , transformation (genetics) , anatomy
Using crown‐gall tumors induced by Agrobacterium tumefaciens strains 181 and EU6 several unusual compounds have been isolated in a single fraction. Physicochemical analysis of the compounds in this fraction showed that they are N 2 ‐(1,3‐dicarboxypropyl)‐L‐amino acids derived from neutral and acidic amino acids. Asparagine and glutamine derivatives (asparaginopine and glutaminopine) are the main components. Synthesis of asparaginopine from asparagine, α‐ketoglutarate and NADPH, and degradation of asparaginopine to asparagine have also been demonstrated using enzyme prepared from Pinto bean strain 181 tumor.