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Identification of barley and wheat cDNA clones related to the high‐ M r polypeptides of wheat gluten
Author(s) -
Forde J.,
Forde B.G.,
Fry R.P.,
Kreis M.,
Shewry P.R.,
Miflin B.J.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80788-1
Subject(s) - endosperm , complementary dna , storage protein , hordein , biology , cdna library , hordeum vulgare , clone (java method) , nucleic acid sequence , gluten , prolamin , microbiology and biotechnology , genetics , gene , botany , poaceae , biochemistry
We have identified 3 cDNA clones related to the high‐ M r group of storage proteins in barley endosperm, the D‐hordeins. A cDNA library has been constructed from wheat endosperm poly(A + )‐RNA and screened using one of the D‐hordein cDNA clones. Two wheat clones which cross‐hybridised to the barley clone have been identified, by hybrid‐release translation and nucleotide sequence analysis, as partial copies of mRNAs encoding the high‐ M r gluten polypeptides of wheat.