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The effect of specific antibodies on oxygen uptake and H + pumping by cytochrome c oxidase vesicles
Author(s) -
Chan Samuel H.P.,
Freedman Jo A.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80785-6
Subject(s) - chemistry , cytochrome c oxidase , vesicle , oxygen , biophysics , cytochrome c , biochemistry , membrane , enzyme , mitochondrion , biology , organic chemistry
Antibodies to solubilized cytochrome c oxidse and to subunit III were incubated with liposomal oxidase. In oxygen uptake experiments, the inhibiting effects on RCI of anti‐oxidase (primarily anti‐ subunits II and IV) and anti‐III were by different mechanisms: the former, by inhibiting the uncoupled rate; the latter, by stimulating the coupled rate. In experiments with H + translocation, anti‐oxidase was without effect, while anti‐III was a potent inhibitor of proton pumping. These results are conclusive evidence for redox‐linked proton extrusion from the vesicles by the oxidase (and its subunit III).