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Multiple environments of fluorinated anesthetics in intact tissues observed with 19 F NMR spectroscopy
Author(s) -
Wyrwicz Alice M.,
Li Yao-En,
Schofield John C.,
Tyler Burt C.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80783-2
Subject(s) - methoxyflurane , anesthetic , halothane , chemistry , nuclear magnetic resonance spectroscopy , chemical shift , spectroscopy , general anesthetics , fluorine 19 nmr , nuclear magnetic resonance , membrane , biophysics , analytical chemistry (journal) , biochemistry , stereochemistry , chromatography , anesthesia , organic chemistry , biology , medicine , physics , quantum mechanics
The incorporation of two fluorine‐containing general anesthetic agents, halothane and methoxyflurane, into erythrocytes (from three different species), rabbit muscle and rabbit nerve, was followed with 19 F NMR spectroscopy. Two major findings emerged from these studies: (1) multiple environments indicative of domain structure in the membrane can be observed depending on the anesthetic and the tissue type; and (2) the 19 F chemical shifts of a given anesthetic were characteristic for the tissue examined. Halothane showed a single resonance in erythrocytes and multiple resonances in muscle and nerve, while methoxyflurane showed multiple resonances in both muscle and erythrocytes. The range of the 19 F chemical shifts for the multiple peaks was as great as 6 ppm.