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Differences in metabolite levels upon differentiation of intact neuroblastoma × glioma cells observed by proton NMR spectroscopy
Author(s) -
Navon Gil,
Burrows Howard,
Cohen Jack S.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80780-7
Subject(s) - neuroblastoma , chemistry , nuclear magnetic resonance spectroscopy , metabolite , proton , proton nmr , glutamine , glioma , spectroscopy , cell culture , biochemistry , biology , stereochemistry , cancer research , amino acid , genetics , physics , quantum mechanics
Proton NMR spectroscopy was used to study the effect of differentiation with prostaglandin E 1 and theophylline on intact hybrid neuroblastoma × glioma cells. The standard proton NMR method showed more resolvable signals than the spin echo NMR spectra. The differentiated cells were found to contain significantly higher levels of glutamine than the undifferentiated precursors. Observations on cell extracts confirmed these results.