Premium
Effect of S‐100 protein on assembly of brain microtubule proteins in vitro
Author(s) -
Donato Rosario
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80778-9
Subject(s) - microtubule , in vitro , microbiology and biotechnology , chemistry , tubulin , biophysics , biochemistry , biology
S‐100 protein inhibits the assembly of brain microtubule proteins in vitro in the presence of 10 μM free Ca 2+ . The S‐100 effect is generally greater on the rate than on the extent of assembly, and even greater as the microtubule protein concentration decreases and the time of preincubation between S‐100 and microtubule proteins before GTP addition increases, at a given S‐100/tubulin dimer molar ratio. The S‐100 effect is greatly enhanced in the presence of physiological concentrations of K + and is completely reversed by EGTA.