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Complementary addressed modification of oligonucleotide d(pGpGpCpGpGpA) with platinum derivative of oligonucleotide d(pTpCpCpGpCpCpTpTpT)
Author(s) -
Vlassov V.V.,
Gorn V.V.,
Ivanova E.M.,
Kazakov S.A.,
Mamaev S.V.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80773-x
Subject(s) - oligonucleotide , guanosine , chemistry , residue (chemistry) , reagent , polynucleotide , stereochemistry , oligonucleotide synthesis , nucleotide , diethylenetriamine , combinatorial chemistry , biochemistry , organic chemistry , dna , gene
New heterobifunctional reagent [BrPt(dien)—(CH 2 ) 6 —(dien)Pt(H 2 O)] 3+ (I) (− dien = diethylenetriamine residue) is proposed for preparation of complementary addressed reagents, reactive derivatives of oligonucleotides and polynucleotides. Two reactive groupings of (I) bind to oligonucleotides at different rates due to the higher reactivity of −[(dien)Pt(H 2 O)] 2+ as compared to −[(dien)Pt Br] + . Using (I), bromodiethylenetriaminoplatinum group was attached to the oligonucleotide d(pTpCpCpGpCpCpTpTpT) (II) by rapid reaction of aquatriaminoplatinum group of (I) with guanosine residue of (II). The reactive oligonucleotide derivative III thus obtained was shown to modify predominantly 5′‐terminal guanosine residue in the complementary oligonucleotide d(pGpGpCpGpGpA) (IV).