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Inhibition of glutamate dehydrogenase activity in rabbit renal mitochondria by phosphoenolpyruvate
Author(s) -
Bryła Jadwiga,
Marzena Matyaszczyk
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80764-9
Subject(s) - phosphoenolpyruvate carboxykinase , glutamate dehydrogenase , biochemistry , glutamate receptor , chemistry , mitochondrion , lactate dehydrogenase , enzyme , dehydrogenase , pyruvate dehydrogenase phosphatase , pyruvate dehydrogenase complex , receptor
The effect of phosphoenolpyruvate on glutamate dehydrogenase activity was studied in both intact and Triton X‐100‐treated rabbit renal mitochondria. The intramitochondrial phosphoenolpyruvate content was modulated by application of both 3‐MPA, an inhibitor of phosphoenolpyruvate carboxykinase, and BTCA, which inhibits the tricarboxylate‐transporting system. The data indicate that: (i) phosphoenolpyruvate is a potent inhibitor of glutamate dehydrogenase activity; and (ii) its inhibitory effect on the enzyme may be abolished by leucine and ADP, activators of glutamate dehydrogenase.