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Occurrence of a new hematoside in the kidney of guinea pig
Author(s) -
Hirabayashi Yoshia,
Li Yu-Teh,
Li Su-Chen
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80744-3
Subject(s) - sialic acid , neuraminidase , periodate , galactose , chemistry , ganglioside , guinea pig , acid hydrolysis , hydrolysis , thin layer chromatography , biochemistry , kidney , chromatography , biology , enzyme , endocrinology
We have isolated a new hematoside from guinea pig kidney. Like the usual hematoside (II 3 NeuAc‐LacCer), isolated from human erythrocytes, this new hematoside contained glucose, galactose, and N ‐acetylneuraminic acid in an equimolar proportion. By thin‐layer chromatography (TLC), however, it migrated faster than the usual hematoside. After mild alkaline hydrolysis the TLC mobility of this ganglioside bacame identical to that of the usual hematoside. The sialic acid in this ganglioside was susceptible to Clostridial neuraminidase. Based on TLC mobility and the results of periodate oxidation, the sialic acid of the new hematoside was identified as 9‐ O ‐acetyl‐ N ‐acetylneuraminic acid. Therefore, the structure of this new hematoside is 9‐ O ‐Ac‐NeuAcα2→3Galβ1→4GLcβ1→1′Cer.