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Effect of acclimation temperature on the concentration of the mitochondrial ‘uncoupling’ protein measured by radioimmunoassay in mouse brown adipose tissue
Author(s) -
Ashwell Margaret,
Jennings Graham,
Richard Denis,
Stirling Dorothy M.,
Trayhurn Paul
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80740-6
Subject(s) - brown adipose tissue , uncoupling protein , radioimmunoassay , adipose tissue , acclimatization , thermogenin , chemistry , medicine , endocrinology , mitochondrion , biology , biochemistry , botany
The effect of acclimation temperature on the concentration of the mitochondrial ‘uncoupling’ protein ( M r 32 000) from brown adipose tissue of mice has been investigated. The uncoupling protein was measured by a specific radioimmunoassay. Between 33°C (thermoneutrality) and −2°C there was a progressive increase with decreasing environmental temperature in the amount of uncoupling protein. For mice at −2°C the mitochondrial concentration of the protein was 9‐times higher than at 33°C, while the total amount of the protein in interscapulaar brown adipose tissue was estimated to be nearly 80‐times greater at −2°C compared to 33°C.