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Direct measurements of the dextran‐dependent calcium uptake by rat peritoneal mast cells
Author(s) -
Sagi-Eisenberg Ronit,
Geller-Bernstein Carmi,
Ben-Neriah Ziva,
Pecht Israel
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80725-x
Subject(s) - phosphatidylserine , calcium , degranulation , dextran , chemistry , ionophore , biophysics , biochemistry , microbiology and biotechnology , biology , membrane , phospholipid , receptor , organic chemistry
The metallochromic indicator murexide has been used to monitor calcium concentration changes during the dextran‐induced, phosphatidylserine‐dependent degranulation of rat peritoneal mast cells. The dextran‐induced Ca 2+ ‐uptake showed an absolute dependence on the presence of phosphatidylserine. The extent of Ca 2+ ‐uptake increased with phosphatidylserine in a concentration‐dependent manner. At 25°C the half‐life of the uptake process equalled 35 ± 5 s. Exposure of the mast cells to dextran in the presence of Ca 2+ , but in the absence of phosphatidylserine, desensitized the cells. The subsequent addition of phosphatidylserine failed to restore the Ca 2+ ‐uptake activity. However, the Ca 2+ ‐ionophore A23187 did promote Ca 2+ uptake by the cells without PS.