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Cloning and expression of the hydrogenase gene from Clostridium butyricum in Escherichia coli
Author(s) -
Karube Isao,
Urano Naoto,
Yamada Takashi,
Hirochika Hirohiko,
Sakaguchi Kenji
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80689-9
Subject(s) - clostridium butyricum , hydrogenase , escherichia coli , plasmid , pbr322 , psti , gene , cloning (programming) , microbiology and biotechnology , chemistry , biology , biochemistry , bacteria , genetics , enzyme , computer science , programming language
Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd − ) using pBR322 and Pst I. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI‐DNA fragments (3.9, 2.6, 0.7, 0.03–0.04, <0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3.1–3.5‐times as high as those of the present strains, such as C. butyricum and E. coli C600 (Hyd + ).