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Monoclonal antibodies inhibiting RNA polymerase from Escherichia coli
Author(s) -
Nikiforov V.G.,
Yakubov L.Z.,
Bogachova G.T.,
Lebedev A.N.,
Rokhlin O.V.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80687-5
Subject(s) - microbiology and biotechnology , monoclonal antibody , rna polymerase , escherichia coli , polymerase , antibody , rna , enzyme , chemistry , protein subunit , rna polymerase i , rna dependent rna polymerase , biology , biochemistry , gene , immunology
Monoclonal hybridoma antibodies directed against RNA polymerase from E. coli have been obtained. Only a few have been found to inhibit the enzyme activity. Antibodies produced by two clones, PYN‐1 and PYN‐2, inhibit RNA polymerase at the stage of RNA chain elongation. the PYN‐1 antibodies react with the β′‐subunit of the enzyme. The PYN‐2 antibodies react with the β‐subunit and with its 130 kDa amber fragment.