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Glutamine synthetase from the plant fraction of Phaseolus root nodules
Author(s) -
Cullimore Julie V.,
Miflin B.J.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80686-3
Subject(s) - phaseolus , root nodule , polysome , glutamine synthetase , biochemistry , sucrose , biology , messenger rna , glutamine , in vitro , rna , chemistry , botany , ribosome , nitrogen fixation , amino acid , gene , bacteria , genetics
Antiserum raised to glutamine synthetase (GS) from the plant fraction of Phaseolus vulgaris root nodules was used to immunoprecipitate in vitro translation products of nodule and root poly(A) + RNA. The results showed that the large increase in GS activity which occurs during nodulation is due to an increased supply of GS mRNA. A 3‐fold enrichment of GS mRNA was obtained by centrifugation of total nodule poly. (A) + RNA on dimethylsulphoxide‐formamide‐containing sucrose density gradients and purification to near homogeneity was achieved by immunopurification of nodule polysomes.