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A third DNA‐dependent ATPase from Bacillus cereus free of ATP‐dependent DNase activity
Author(s) -
Ohlbaum A.,
Csuzi S.,
Antoni F.,
Banfalvi G.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80677-2
Subject(s) - nuclease , atpase , dna , enzyme , biochemistry , bacillus cereus , microbiology and biotechnology , atp hydrolysis , deoxyribonuclease , biology , chemistry , bacteria , genetics
The purification of ATP‐dependent DNase from Bacillus cereus led to the isolation and characterization of a third DNA‐dependent ATPase. The enzyme called ATPase III has been purified free of nuclease activity. None of the expected ATPases proved to be identical with ATP‐dependent DNase‐DNA‐dependent ATPase. Separation of ATPase I, II and III and a DNase specific for single‐stranded DNA from the same source excludes the possibility of ATP‐dependent DNase being the action of a single enzyme molecule.