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Karyoduction in Saccharomyces cerevisiae
Author(s) -
Maemura Tohru,
Yamashita Ichiro,
Fukui Sakuzo
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80674-7
Subject(s) - ethidium bromide , saccharomyces cerevisiae , protoplast , yeast , dapi , tetrad , strain (injury) , respiration , polyethylene glycol , staining , saccharomyces , chemistry , biochemistry , cytoplasm , biology , dna , genetics , botany , anatomy
We introduced the nuclei isolated from the respiration‐sufficient killer strain of the yeast Saccharomyces cerevisiae into the yeast protoplasts prepared from the respiration‐deficient non‐killer strain with the aid of polyethylene glycol. The resulting karyoductants were respiration‐deficient non‐killers. Nuclear staining with ethidium bromide or DAPI and tetrad analysis of the karyoductants presented evidence that the nuclei introduced into protoplasts were fused to the resident nuclei, leading to stable diploids. This technique termed karyoduction will be useful in the study of nucleo—cytoplasmic relationship in yeast and other organisms.