Premium
Selective inhibition of 5‐lipoxygenase by natural compounds isolated from Chinese plants, Artemisia rubripes Nakai
Author(s) -
Koshihara Yasuko,
Neichi Tomohiro,
Murota Sei-itsu,
Lao Ai-na,
Fujimoto Yasuo,
Tatsuno Takashi
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80672-3
Subject(s) - caffeic acid , chemistry , biochemistry , artemisia , arachidonate 5 lipoxygenase , arachidonic acid , biology , botany , enzyme , antioxidant
Three of four natural compounds, which are caffeic acid, eupatilin and 4′‐demethyleupatilin, isolated from Chinese plant, Artemisia rubripes Nakai selectively inhibited 5‐lipoxygenase of cultured mastocytoma cells. Half‐inhibition doses ( ID 50 ) for caffeic acid, eupatilin and 4′‐demethyleupatilin were 3.7, 14 and 18 × 10 −6 M, respectively. The inhibition by caffeic acid was non‐competitive types. Prostaglandin synthase activities were little inhibited by eupatilin and 4′‐demethyleupatilin, but rather stimulated by affeic acid. The formation of leukotriene C 4 and D 4 by mast tumor cells was almost completely suppressed by these compounds at 10 −4 M.