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Liposome‐mediated labeling of adrenocorticotropin fragments parallels their biological activity
Author(s) -
Gysin Beat,
Schwyzer Robert
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80666-8
Subject(s) - chemistry , diazirine , liposome , in vitro , photoaffinity labeling , membrane , biochemistry , receptor
To test our hypothesis that specific interactions of ACTH peptides with model lipid membranes reflect the biological importance of similar interactions on target cells, we investigated the liposome‐mediated labeling of ACTH fragments with the extremely hydrophobic photolabel, 3‐trifluoromethyl‐3‐( m ‐[ 125 I]iodophenyl)diazirine. Correlations were found between the labeling rates and the agonistic and antagonistic potencies of the peptides for in vitro steroidogenesis and inhibition of a synaptosomal protein kinase. A model for the cross‐reactivity between ACTH and opioid peptides is discussed.