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Biosynthesis of rabbit haptoglobin: Chemical evidence for a single chain precursor
Author(s) -
Chow Virginia,
Murray Robert K.,
Dixon James D.,
Kurosky Alexander
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80623-1
Subject(s) - rabbit (cipher) , haptoglobin , biosynthesis , chemistry , biochemistry , chain (unit) , biology , immunology , enzyme , computer science , physics , computer security , astronomy
The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell‐free system by immunoprecipitation with an antiserum that was directed to the β chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of M r 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the α chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by α and β chain regions, respectively.