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Influence of hyperthermia on the phosphorylation of ribosomal protein S6 from human skin fibroblasts and meningioma cells
Author(s) -
Richter W.W.,
Zang K.D.,
Issinger O.-G.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80620-6
Subject(s) - dephosphorylation , ribosomal protein s6 , phosphorylation , ribosome , ribosomal protein , ribosomal rna , hyperthermia , incubation , microbiology and biotechnology , cell culture , fibroblast , chemistry , biology , protein phosphorylation , biochemistry , rna , phosphatase , gene , genetics , protein kinase a , paleontology
Skin fibroblasts and meningioma cells, derived from primary cultures of the same patients have been used to study the influence of hyperthermia on (i) cell morphology and (ii) phosphorylation pattern of ribosomal and ribosome‐associated proteins. Incubation of tumour cells and fibroblasts up to 7 h at 42°C did not significantly change the cell morphology as compared to control cells kept at 37°C. At 42°C ribosomal protein S6 is shifted cathodically indicating a loss of negative charge, however no quantitative dephosphorylation of S6 was observed. Meningioma cells and fibroblasts did not differ significantly with respect to S6 phosphorylation.