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Isolation and characterization of 15‐hydroxylated metabolites of leukotriene C 4
Author(s) -
Örning Lars,
Hammarström Sven
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80618-8
Subject(s) - metabolite , chemistry , leukotriene , lipoxygenase , biochemistry , stereochemistry , chromatography , leukotriene d4 , high performance liquid chromatography , enzyme , biology , antagonist , asthma , immunology , receptor
A polar metabolite of leukotriene C 4 was formed by sequential conversions with soybean lipoxygenase I and liver peroxidase. The structure of this product was found to be 5( S ), 15( S )‐dihydroxy‐6( R )‐ S ‐glutathionyl‐7,9,13‐ trans ‐11‐ cis ‐eicosatetraenoic acid (15‐hydroxy‐Δ 13 ‐ trans ‐leukotriene C 3 ). The HPLC behaviour, the molar extinction coefficient and the biological activity of the metabolite are reported. Preliminary evidence suggests that this product is formed by mammalian tissues.