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A calcium‐activated nuclease endogenous to Xenopus erythrocytes
Author(s) -
Talwar Sandeep,
Pocklington Michael J.,
Maclean Norman
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80611-5
Subject(s) - xenopus , nuclease , endogeny , chemistry , calcium , microbiology and biotechnology , biophysics , biochemistry , biology , enzyme , gene , organic chemistry
An endogenous nuclease is found in purified Xenopus erythrocyte nuclei. This enzyme is activated specifically by Ca 2+ in the presence of which chromatin is digested into discrete fragments. Both 10 bp and 200 bp ladders are detected upon electrophoresis of DNA. This endogenous enzyme therefore resembles mammalian and chicken liver endonucleases.