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Fluorescence study of secondary structure of DNA within bacteriophage λ
Author(s) -
Shurdov M.A.,
Kiyanov S.V.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80597-3
Subject(s) - dna , bacteriophage , fluorescence , tryptophan , chemistry , protein secondary structure , biophysics , microbiology and biotechnology , biochemistry , biology , amino acid , escherichia coli , gene , physics , quantum mechanics
Bromoacetaldehyde (BAA) was used to study the secondary structure of DNA in λ‐phage particles. It was determined that about 1% of the adenines in the intraphage λ‐DNA reacts readily with BAA, thus, they are placed in DNA sites with disturbed complementary interactions. These adenines are close to the tryptophan residues of the phage protein. Fluorescence emission of ϵA in the intraphage DNA is dramatically quenched. This, apparently, indicates the interaction between ϵA and Trp‐ and/or Tyr‐ and/or Met‐residues of phage protein.