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Site of tubulin synthesis in Tetrahymena pyriformis
Author(s) -
Barahona Isabel,
Rodrigues-Pousada Claudina
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80593-6
Subject(s) - polysome , reticulocyte , tubulin , tetrahymena pyriformis , protein biosynthesis , polyadenylation , biology , biochemistry , protozoa , rna , microbiology and biotechnology , chemistry , tetrahymena , microtubule , ribosome , genetics , gene
Free and membrane‐bound polysomes were isolated from the protozoa Tetrahymena pyriformis , and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35 S‐translational products in a rabbit reticulocyte lysate. One‐dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane‐bound polysomes. Non‐polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two‐dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α‐ and β‐tubulin subunits.