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Identification of antigenic components of Toxoplasma gondii by an immunoblotting technique
Author(s) -
Partanen Paul,
Turunen Hannu J.,
Paasivuo Raili,
Forsblom Erik,
Suni Jukka,
Leinikki Pauli O.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80589-4
Subject(s) - toxoplasma gondii , antigen , antibody , microbiology and biotechnology , peroxidase , polyacrylamide gel electrophoresis , biology , blot , gel electrophoresis , chemistry , electrophoresis , biochemistry , enzyme , immunology , gene
Proteins of Toxoplasma gondii were separated by SDS‐polyacrylamide gel electrophoresis with subsequent transfer to a nitrocellulose sheet by electrophoretic blotting. Immunologically reactive polypeptides were detected by human sera with previously known toxoplasma antibody levels. Heavy chain‐specific, peroxidase‐conjugated anti‐human immunoglobulins were used as the indicator antibodies for the separate identification of IgG and IgM reactive polypeptides. IgG toxoplasma antibodies reacted with several antigens of M r ≈27 000–67 000, while toxoplasma‐specific IgM seemed to detect only a few polypeptides. The M r of 35 000 for the dominating IgM reactive polypeptide was observed.