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Membrane dynamics in human leukemia and lymphoma cells
Author(s) -
Billard C.,
Phan Dinh Tuy F.,
Rosenfeld C.,
Tapiero H.
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80584-5
Subject(s) - diphenylhexatriene , glutaraldehyde , chemistry , fluorescence anisotropy , phospholipid , cell culture , biochemistry , membrane , biophysics , leukemia , lymphoma , sodium azide , protease , enzyme , chromatography , biology , immunology , genetics
Membrane dynamics of human leukemia and lymphoma cell lines were analyzed by investigating the effect of pH on fluorescence polarization ( P ) of the lipophilic probe diphenylhexatriene (DPH). The degree of P varied as a function of pH, depending on the cell lines. These variations were not detected in phospholipid vesicles. In addition, they were prevented by treatments with glutaraldehyde, sodium azide or phenylmethylsulfanyl fluoride, a specific protease inhibitor. Therefore, these P value changes might be influenced by protein modification.