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[2,3‐ 3 H]Propylhydroxylamine inhibits ATP synthesis of TF 1 F o liposomes in proton jump experiments
Author(s) -
Skrzipczyk Heinz Jürgen,
Küchler Bernd,
Bäuerlein Edmund
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80572-9
Subject(s) - protonation , chemistry , proton , liposome , hydroxylamine , stereochemistry , medicinal chemistry , crystallography , ion , biochemistry , physics , organic chemistry , quantum mechanics
ATP synthesis of TF 1 ·F o liposomes in proton‐jump experiments was inhibited by N ‐[2,3‐ 3 H]propylhydroxylamine. High radioactivity was shown by SDS‐PAGE to be incorporated into the proteolipid. The activation of one of its carboxyl groups, which was apparently trapped by the hydroxylamine derivative, may proceed by the protonation of an undissociated carboxyl group as part of the proton translocation. If ADP was omitted, no trapping reaction occurred. Thus binding of ADP to TF 1 appeared to be necessary for such an activation of a carboxyl group.