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Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter
Author(s) -
Bækkeskov Steinunn,
Lernmark Åke
Publication year - 1983
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(83)80570-5
Subject(s) - antiserum , islet , transporter , biochemistry , chemistry , biosynthesis , medicine , endocrinology , pancreatic islets , biology , diabetes mellitus , antibody , gene , immunology
An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of M r 36 000 and a minor protein of M r 58 000 from human pancreatic islets. The biosynthetic incorporation of [ 35 S]methionine into the 36‐kDa protein increased 2–3 fold in 16 mM D‐glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D‐glucose either with or without IBMX. D‐Glucose tested without or with IBMX had no effect on the biosynthesis of the 58‐kDa protein. The 36‐kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D‐glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic β‐cell.